Journal article
Kidney organoids from human iPS cells contain multiple lineages and model human nephrogenesis
M Takasato, PX Er, HS Chiu, B Maier, GJ Baillie, C Ferguson, RG Parton, EJ Wolvetang, MS Roost, SMC De Sousa Lopes, MH Little
Nature | NATURE PUBLISHING GROUP | Published : 2015
DOI: 10.1038/nature15695
Abstract
The human kidney contains up to 2 million epithelial nephrons responsible for blood filtration. Regenerating the kidney requires the induction of the more than 20 distinct cell types required for excretion and the regulation of pH, and electrolyte and fluid balance. We have previously described the simultaneous induction of progenitors for both collecting duct and nephrons via the directed differentiation of human pluripotent stem cells. Paradoxically, although both are of intermediate mesoderm in origin, collecting duct and nephrons have distinct temporospatial origins. Here we identify the developmental mechanism regulating the preferential induction of collecting duct versus kidney mesenc..
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Awarded by Australian Research Council
Funding Acknowledgements
This research was supported by National Health and Medical Research Council (NHMRC) of Australia (APP1041277, APP1037320), Australian Research Council (ARC) (SRI110001002, CE140100036), Bontius Stiching and Organovo Inc. M.H.L. and R.G.P. are NHMRC Senior Principal Research Fellows. B.M. is a Rosamond Siemon Postgraduate Scholar. We thank A. Christ and T. Bruxner at the IMB Sequencing Facility for providing NGS service. We also acknowledge the IMB ACRF Imaging Facility and the Australian Microscopy & Microanalysis Research Facility at the Center for Microscopy and Microanalysis at The University of Queensland.